REFERENCE

논문 제목	Development of antibodies against severe fever with thrombocytopenia syndrome virus nucleoprotein for diagnosis
저자	Eun-Mee Park, Seheon Kim, Seoyoen Lim, Parastou Rahimizadeh, Hyeyoon Jeon, Hyung Jin Lim, Sungjin Lee, Yong Bhum Song
저널	Applied Microbiology and Biotechnology
출판연도	2025
PMC	PMC12145327
DOI	https://doi.org/10.1007/s00253-025-13530-1

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral infectious disease caused by a novel Bandavirus in the family Phenuiviridae. The SFTS virus (SFTSV) is transmitted to various hosts, including humans, through tick bites, leading to high fever, thrombocytopenia, and leukopenia, with a high case fatality rate (up to 30%) due to multiple organ dysfunction. Therefore, early diagnosis is crucial for effective treatment and preventing disease transmission. In this study, we aimed to develop and characterize monoclonal antibodies targeting the SFTSV nucleocapsid protein (NP). We generated recombinant NP to screen antibodies against SFTSV. Using phage display technology, we identified candidate single-chain variable fragment (scFv) sequences capable of detecting SFTSV NP. Five human IgG antibodies and six chimeric mouse antibodies exhibited strong binding ability to the recombinant NP. Furthermore, their specificity and selectivity were evaluated against NPs from different subtypes and other viral species. A sandwich enzyme-linked immunosorbent assay (ELISA) was performed to determine optimal antibody pairings for SFTSV detection. The mP01A05/hP01C09, mP01A05/hP01B10, and mP02E04/hP01A05 antibody pairs demonstrated high efficacy in diagnosing SFTSV infections.

요약

치명률이 최대 30%에 달하는 중증열성혈소판감소증후군(SFTS, 살인진드기 감염병) 바이러스의 조기 진단을 위해, SFTSV 뉴클레오캡시드 단백질(NP)에 특이적인 단클론 항체를 파지 디스플레이 기술로 개발한 연구입니다. YntoAb의 파지 라이브러리를 활용하여 인간 IgG 항체 5종 및 키메릭 마우스 항체 6종을 생산하고, 샌드위치 ELISA 최적 항체 쌍을 규명하여 SFTS 임상 진단 플랫폼 구축을 위한 항체 자원을 확보하였습니다.

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